A novel, safe, accurate and sensitive spectrophotometric method was developed using hydrotropes mixture (10 gm niacinamide in 25 ml phenol) as hydrotropic solubilizing agent for the determination of clofazimine. There were more than 70 fold enhancements in the solubility of clofazimine increases in hydrotropes mixture as compared to solubilities in distilled water. Clofazimine shows maximum absorbance at 499 nm. Hydrotropes mixture did not show any absorbance above 300 nm and thus no interferences were seen. Clofazimine obeyed beer’s law in the concentration range of 5-25 μg/ml (r2= 0.9998) in hydrotropes mixture with means recovery 99.76 %. Proposed method is new, simple, economic, safe, rapid, accurate and reproducible. The developed methods were validated according to ICH guideline Q2 (R1) and values of accuracy, precision and other statistical analysis were found to be in good accordance with prescribed values.
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